The Resolute® BioSC platform can be a highly modular multi-action chromatography system which will consistently run a few chromatography separations (in batch or multi-column mode), which include viral inactivation and in-line buffer preparing. The chaining of several device operations with each other ends in a compact and intensified approach.
Ion-exchange: Separates charged molecules based mostly on their conversation with charged purposeful groups about the stationary phase.
측정 가능한 농도 범위는 컬럼에 의해서도 결정됩니다. 컬럼 충진제의 종류, 입자 지름, 컬럼의 크기에 따라 분리에 최적인 시료 주입량이 크게 다릅니다.
High-Performance Liquid Chromatography (HPLC) is a sophisticated analytical approach based upon chromatographic principles of separation and interaction concerning substances and stationary and cell phases.
Degassing is achieved in several methods, but the commonest are using a vacuum pump or sparging using an inert gas, including He, which has a low solubility from the cell period. Particulate materials, which can clog the HPLC tubing or column, are taken off by filtering the solvents.
カラム周辺の温度の変動によって溶出時間が安定せず再現性が悪くなる場合があるため、カラム温度を一定に保つために使用する。またカラム温度を分離条件のパラメーターの一つとして積極的に利用する場合もある。
混合物で構成される試料を分離する。一般にステンレス製の筒の中に、微細な真球状の多孔質シリカゲルをアルキル基等で修飾した物を充填して用いる。分取目的であれば、粉砕シリカゲルも用いられる。
前述した従来の順相タイプに対して、逆相クロマトグラフィーにおいては固定相に低極性のもの（例えばシリカゲルにアルキル基を共有結合させたもの）を、移動相に高極性のもの（例えば水や塩類の水溶液、アルコール、アセトニトリルなどの有機溶媒）を用いる。また珍しいケースではあるが、分離のための移動相pHをシリカゲルの使用範囲から外れたところに設定する必要がある場合、あるいはシリカゲル表面に残っている未反応シラノール基が分離に悪影響を及ぼし、かつそれが移動相の変更によっても解決できない場合には、固定相として樹脂を用いることがある。分析物はより極性の低いほどより強く固定相と相互作用して溶出が遅くなる。また極性の低い物質の割合が多い移動相ほど溶出が早くなる。
Polarity: The polarity on the cell phase noticeably influences separation. A far more polar cell stage interacts much more strongly with polar analytes, producing them to elute (exit the column) slower than much less polar analytes.
The dimensions with the particles and the mechanical energy on the packing products read more are the two vital things that have an effect on column packing. The particle might be packed and dried if much larger than 20 mm, however, if scaled-down than 20 mm, it need to be suspended in the suitable solvent. The slurry is then packaged.
The cell phase’s circulation charge is determined by the put together speeds of The 2 pumps. By altering the relative speeds of The 2 pumps, different binary mobile phases could be well prepared.
, for instance, shows more info retention periods for four weak acids in two cell phases with approximately equivalent values for (P^ primary ). Although the purchase of elution is identical for each cellular phases, each solute’s retention time is affected in different ways by the selection of natural solvent.
To attenuate these difficulties we position a guard column ahead of the analytical column. A Guard column usually consists of the same particulate packing product and stationary phase as the analytical column, but is significantly shorter and less expensive—a duration of 7.five mm and a cost just one-tenth of that with the corresponding analytical column is usual. Because they are intended to be sacrificial, guard columns are replaced regularly.
A quantitative HPLC Examination is usually less complicated than a quantitative GC Assessment due to the fact a hard and fast volume sample loop presents a far more specific and correct injection.